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caspase 12  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology caspase 12
Caspase 12, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polypropylene+columns+product+number+29924/caspase-12+siRNA/pmc03015155-287-5-13
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epsin 1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology epsin 1
Fig. 3. (A) Translocation of AT2 and PLZF by Ang II stimulation in CHO-K1 cells transfected with AT2 and PLZF. Green fluorescence (EGFP) indicates AT2 or AT1, red fluorescence (rhodamine Red-X) indicates anti-myc (PLZF). CHO-K1 cells were transfected and treated as indicated. Control means without Ang II treatment, and indicated time refers to the duration of Ang II exposure. The green and red channels were used separately for the first row and the second row to visualize AT2 and PLZF separately in the same cells. The third row is the overlay. (B) The nuclear localization of PLZF (anti-myc) in early stage R3T3 cells transfected with PLZF and stimulated with Ang II for 60 min. RB is used as the nuclear marker. (C) Percentage decrease of AT2-binding sites on the cell surface using [125I]Ang II in early stage R3T3 cells transfected with PLZF. Indicated time refers to the duration of Ang II exposure. Data are representative of three independent experiments with nearly identical results. (D) PLZF is associated with <t>epsin</t> <t>1</t> before Ang II stimulation. CHO-K1 cells were transfected with AT2 and PLZF. Green fluorescence (EGFP) indicates PLZF, red fluorescence (rhodamine Red-X) indicates anti-epsin. Control is without Ang II treatment, and indicated time refers to the duration of Ang II exposure. Immunoprecipitation was performed with anti-GFP and then western blot was performed with anti-epsin. (E) AT2–PLZF complex did not involve β2-arrestin. CHO-K1 cells were transfected with AT1 alone, or AT2 and PLZF. Green fluorescence (EGFP) indicates AT1 or AT2, red fluorescence (rhodamine Red-X) indicates anti-β2-arrestin. Control is without Ang II treatment, and indicated time refers to the duration of Ang II exposure.
Epsin 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polypropylene+columns+product+number+29924/epsin+1+Antibody/pmc00291832-542-16-21
Average 93 stars, based on 1 article reviews
epsin 1 - by Bioz Stars, 2026-09
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Image Search Results


Fig. 3. (A) Translocation of AT2 and PLZF by Ang II stimulation in CHO-K1 cells transfected with AT2 and PLZF. Green fluorescence (EGFP) indicates AT2 or AT1, red fluorescence (rhodamine Red-X) indicates anti-myc (PLZF). CHO-K1 cells were transfected and treated as indicated. Control means without Ang II treatment, and indicated time refers to the duration of Ang II exposure. The green and red channels were used separately for the first row and the second row to visualize AT2 and PLZF separately in the same cells. The third row is the overlay. (B) The nuclear localization of PLZF (anti-myc) in early stage R3T3 cells transfected with PLZF and stimulated with Ang II for 60 min. RB is used as the nuclear marker. (C) Percentage decrease of AT2-binding sites on the cell surface using [125I]Ang II in early stage R3T3 cells transfected with PLZF. Indicated time refers to the duration of Ang II exposure. Data are representative of three independent experiments with nearly identical results. (D) PLZF is associated with epsin 1 before Ang II stimulation. CHO-K1 cells were transfected with AT2 and PLZF. Green fluorescence (EGFP) indicates PLZF, red fluorescence (rhodamine Red-X) indicates anti-epsin. Control is without Ang II treatment, and indicated time refers to the duration of Ang II exposure. Immunoprecipitation was performed with anti-GFP and then western blot was performed with anti-epsin. (E) AT2–PLZF complex did not involve β2-arrestin. CHO-K1 cells were transfected with AT1 alone, or AT2 and PLZF. Green fluorescence (EGFP) indicates AT1 or AT2, red fluorescence (rhodamine Red-X) indicates anti-β2-arrestin. Control is without Ang II treatment, and indicated time refers to the duration of Ang II exposure.

Journal:

Article Title: A novel angiotensin II type 2 receptor signaling pathway: possible role in cardiac hypertrophy

doi: 10.1093/emboj/cdg637

Figure Lengend Snippet: Fig. 3. (A) Translocation of AT2 and PLZF by Ang II stimulation in CHO-K1 cells transfected with AT2 and PLZF. Green fluorescence (EGFP) indicates AT2 or AT1, red fluorescence (rhodamine Red-X) indicates anti-myc (PLZF). CHO-K1 cells were transfected and treated as indicated. Control means without Ang II treatment, and indicated time refers to the duration of Ang II exposure. The green and red channels were used separately for the first row and the second row to visualize AT2 and PLZF separately in the same cells. The third row is the overlay. (B) The nuclear localization of PLZF (anti-myc) in early stage R3T3 cells transfected with PLZF and stimulated with Ang II for 60 min. RB is used as the nuclear marker. (C) Percentage decrease of AT2-binding sites on the cell surface using [125I]Ang II in early stage R3T3 cells transfected with PLZF. Indicated time refers to the duration of Ang II exposure. Data are representative of three independent experiments with nearly identical results. (D) PLZF is associated with epsin 1 before Ang II stimulation. CHO-K1 cells were transfected with AT2 and PLZF. Green fluorescence (EGFP) indicates PLZF, red fluorescence (rhodamine Red-X) indicates anti-epsin. Control is without Ang II treatment, and indicated time refers to the duration of Ang II exposure. Immunoprecipitation was performed with anti-GFP and then western blot was performed with anti-epsin. (E) AT2–PLZF complex did not involve β2-arrestin. CHO-K1 cells were transfected with AT1 alone, or AT2 and PLZF. Green fluorescence (EGFP) indicates AT1 or AT2, red fluorescence (rhodamine Red-X) indicates anti-β2-arrestin. Control is without Ang II treatment, and indicated time refers to the duration of Ang II exposure.

Article Snippet: The cells were stained by incubation for 1 h at room temperature with antibody against c-myc, epsin 1, β 2 -arrestin (Santa Cruz Biotechnology) or AT 2 at 1:50 dilution in 1% BSA in PBS.

Techniques: Translocation Assay, Transfection, Fluorescence, Marker, Binding Assay, Immunoprecipitation, Western Blot